张全全;谢敏杰;喻志源;王伟;

• 1:华中科技大学同济医学院附属同济医院神经内科

摘要(Abstract)：

目的:建立双侧颈总动脉狭窄(BCAS)所致慢性低灌注脑白质损伤小鼠模型。方法:C57BL/6J小鼠42只,随机分为假手术组和模型组各21只,建立慢性低灌注脑白质损伤模型,假手术组不造成狭窄,利用7T超高场显微磁共振、髓鞘染色和电镜技术检测白质区病变,利用八臂迷宫行为学进行认知功能评判。结果:DTI相关参数显示,代表白质纤维完整性的部分各向异性在模型组的视束、内囊、胼胝体等白质区明显下降(P<0.05),代表脱髓鞘损伤的垂直扩散向量在胼胝体区明显升高(P<0.05),代表轴突损伤的轴向扩散向量在各白质区均未见明显变化;LFB染色和电镜亦证明白质区有明显脱髓鞘损伤。行为学试验证明模型组有明显的工作记忆错误(P<0.001),无明显参考记忆错误,且工作记忆错误和DTI参数所示脑白质损伤之间明显相关(P<0.05)。结论:小鼠BCAS 30 d可以造成选择性白质损伤,并检测到明显的工作记忆损害,两者之间存在相关性。

关键词(KeyWords)： 双侧颈总动脉狭窄;血管性认知功能障碍;低灌注;脑白质损伤

Abstract:

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基金项目(Foundation):

作者(Author): 张全全;谢敏杰;喻志源;王伟;

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参考文献(References)：

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