结核性脑膜炎患者脑脊液S100B与一氧化氮合酶的相关性研究Clinical Significance of S100B and iNOS in Cerebrospinal Fluid from Patients with Tuberculous Meningitis
徐长云,王贤军
摘要(Abstract)：
目的:探讨结核性脑膜炎患者脑脊液S100B、一氧化氮合酶(iNOS)含量的动态变化及两者的相关性。方法:43例结核性脑膜炎患者(TM组)和无神经系统疾病的患者28例(对照组)均于发病后1-6 d首次采集脑脊液,以后每隔5-7 d采集1次,共5次。采用酶联免疫吸附试验(ELISA)、化学比色法分别测定2组患者不同病程脑脊液S100Bi、NOS含量。结果:发生昏迷(昏迷组)15例,未发生昏迷(无昏迷组)28例。昏迷组和无昏迷组不同病程脑脊液S100Bi、NOS含量均较对照组显著增高(P<0.05);昏迷组脑脊液S100Bi、NOS含量较无昏迷组明显增高(P<0.05);昏迷组和无昏迷组患者脑脊液S100B与iNOS含量均呈高度相关(P=0.0319,0.0337)。结论:脑脊液S100B作为脑损害的标志物,与iNOS高度相关,其含量的高低能够反映结核性脑膜炎患者病情的严重程度。
关键词(KeyWords)： S100B;iNOS;脑脊液;结核性脑膜炎
基金项目(Foundation):
作者(Author): 徐长云,王贤军
参考文献(References)：
- [1]SEN J,BELLI A,PETZOLD A,et al.ExtracellularFluid S100B in the Injured Brain:A Future SurrogateMarker of Acute Brain Injury[J]?Acta Neurochir(Wien)(S0001-6268),2005,147(8):897-900.
- [2]PELINKA LE,SZALAY L,JAFARMADAR M,etal.Circulating S100B Is Increased after Bilateral Fe-mur Fracture without Brain Injury in the Rat[J].Br JAnaesthesia(S0007-0912),2003,91(4):595-597.
- [3]DE GIROLAMO P.Introduction[J].Microsc ResTech(S1059-910X),2003,60:537-539.
- [4]HESSIAN A,FISHE L.The Hoterodimerica Complexof MRP-8(S100A8)and MRP-14(S100A9)AntibodyRecognition,Epitope Definition and the Implicationsfor Structure[J].Eur J biochem(S0014-2956),2001,268(2):350-363.
- [5]PORTELA LV,TORT AB,WALZ R,et al.Interic-tal Serum S100B Levels in Chronic Neurocystic-ercosisand Idiopathic Epilepsy[J].Acta Neurol Scand(S0001-6314),2003,108(6):424-427.
- [6]GAZZOLO D,FLORIO P,CIOTTI S,et al.S100BProtein in Urine of Preterm Newborns with OminousOutcome[J].Pediatr Res(S0031-3998),2005,58(6):1170-1174.
- [7]ASANO T,MORI T,SHIMODA T,et al.ArundicAcid(ONO-2506)Ameliorates Delayed Ischemic BrainDamage by Preventing Astrocytic Overproduction ofS100B[J].Curr Drug Targets CNS Neurol Disord(S1568-007X),2005,4(2):127-142.
- [8]MARKOWITZ J,MACKERELL AD,CARRIER F,et al.Design of Inhibitors for S100B[J].Curr TopMed Chem(S1568-0266),2005,5(12):1093-1108.
- [9]FOERCH C,SINGER OC,NEUMANN-HAEFELINT,et al.Evaluation of Serum S100B as a SurrogateMarker for Long-term Outcome and Infarct Volume inAcute Middle Cerebral Artery Infarction[J].ArchNeurol(S0003-9942),2005,62(7):1130-1134.