刘志广,康慧聪,李丽红,李巷,刘建林,曾铮,朱遂强

• 1:华中科技大学同济医学院附属同济医院神经内科

摘要(Abstract)：

目的:观察腺苷A1受体基因敲除小鼠戊四氮致痫后海马神经细胞线粒体功能的损伤。方法:腺苷A1受体基因敲除小鼠和野生型小鼠各30只,各随机分为对照组、致痫后6h、24h、7d、30d组,每组6只;戊四氮点燃制作癫痫模型;于各时间点取小鼠海马,流式细胞仪测线粒体膜电位,免疫荧光测细胞色素C(cyt C)的表达。结果:基因敲除小鼠和野生型小鼠致痫后6h、24h、7d及30d组的线粒体膜电位均低于对照组(P<0.05),cyt C释放量均高于对照组(P<0.05);除对照组外,基因敲除小鼠致痫后6h、24h、7d及30d组的线粒体膜电位均低于野生型小鼠(P<0.05),cyt C释放量均高于野生型小鼠(P<0.05)。结论:癫痫早期即可出现海马线粒体功能损伤,腺苷A1受体有助于减轻癫痫小鼠海马线粒体损伤。

关键词(KeyWords)： 腺苷A1受体;癫痫;海马;线粒体膜电位;细胞色素C

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(No.30770752)

作者(Author): 刘志广,康慧聪,李丽红,李巷,刘建林,曾铮,朱遂强

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