孙明明,张双悦,王秋颖,荆瀛黎,白帆,于艳

• 1:中国康复科学所
• 2:首都医科大学康复医学院
• 3:北京脑重大疾病研究院神经损伤与修复研究所
• 4:神经损伤与康复北京市重点实验室

摘要(Abstract)：

目的：探讨负载槲皮素（quercetin,QR）的含环醚侧基聚己内酯-聚乙二醇三嵌段共聚物（PECT）温敏水凝胶对大鼠脊髓损伤（spinal cord injury,SCI）的修复作用。方法：制备负载QR且37℃条件下自动成胶的温敏水凝胶（QR-polycaprolactone-polyglycol triblock,QR-PECT）；表征水凝胶的温敏及流变特性；通过CCK-8实验评价水凝胶的细胞相容性；通过1,1-二苯基-2-吡啶并肼基（1,1-diphenyl-2-picrylhydrazyl radical,DPPH）自由基清除实验来评估水凝胶清除自由基的能力；通过活性氧（reactive oxygen species,ROS）检测试剂盒评价水凝胶清除细胞内ROS的能力；制备大鼠SCI全切模型，分为Sham组、SCI组、QR治疗组及QR-PECT水凝胶治疗组，应用BBB评分评估大鼠运动功能的恢复，应用尼氏染色观察神经元迁移浸润情况，通过HE染色评价水凝胶生物相容性。结果：制备的QR-PECT聚合物在37℃条件下形成水凝胶，并持续释放QR;CCK-8实验定量结果显示QR-PECT水凝胶治疗组与SCI组的细胞活力相当；DPPH清除实验显示，QR-PECT水凝胶治疗组的DPPH清除率达到60%，与QR治疗组相当；通过测量HT22细胞内的ROS荧光值，发现QR-PECT水凝胶治疗组的HT22细胞内荧光强度显著降低；SCI后8周，QR-PECT水凝胶治疗组大鼠运动功能明显恢复，尼氏染色显示QR-PECT水凝胶治疗组存在大量神经元浸润，HE染色结果显示QR-PECT水凝胶治疗组心、肝、脾、肺、肾与正常大鼠没有明显的差异。结论：QR-PECT水凝胶具有良好的生物相容性和抗氧化性，并能够促进大鼠SCI后神经元的再生和运动功能恢复。

关键词(KeyWords)： 脊髓损伤;槲皮素;QR-PECT水凝胶;氧化性

Abstract:

Keywords:

基金项目(Foundation): 中央级公益性科研院所基本科研业务费专项资金项目（2021CZ-9)

作者(Author): 孙明明,张双悦,王秋颖,荆瀛黎,白帆,于艳

DOI: 10.16780/j.cnki.sjssgncj.20240003

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